What Is Peptide Synthesis?
Peptide synthesis is the process of forming a peptide bond between two amino acids to form a peptide sequence. It involves coupling the carboxyl group of one amino acid to the amino group of another amino acid through the formation of a peptide bond. The synthesis of peptides is mainly divided into chemical synthesis of peptides and biosynthesis of peptides. At Alfa Chemistry, we mainly use chemical synthesis, which takes place in the form of amino acid to amino acid condensation.
In addition, thanks to the advances in computational chemistry methods, peptide synthesis reactivity prediction, synthetic route design, and automated peptide synthesis platforms can be realized by training effective deep learning methods. For example, a case of deep learning used to predict and optimize fast-flowing peptide synthesis is shown in the figure.
Deep learning to predict and optimize fast-flow peptide synthesis.
Our Peptide Synthesis Services
Alfa Chemistry's peptide synthesis experts are dedicated to meeting your peptide synthesis needs, from peptides found in biological samples to unique peptides that require innovative design. Our automated peptide synthesis services involve computer-aided peptide design, automated peptide synthesis, and peptide purification.
- Peptide Design
On the one hand, considering the specific application and customer needs of the peptide, Alfa Chemistry can design the peptide from de novo or based on the peptide sequences of the natural protein. Our synthetic chemists will comprehensively consider the key factors affecting peptide synthesis to tailor peptide synthesis routes and methods for you. These factors may include peptide length, polypeptide solubility, polypeptide composition, polypeptide modifications, etc.
On the other hand, relying on a powerful computational chemistry platform and machine learning methods, Alfa Chemistry can realize computer-aided peptide design services, such as synthetic reactivity prediction, retrosynthetic route planning, reaction condition screening and optimization, etc.
- Automated Peptide Synthesis
The classic methods of chemical peptide synthesis include liquid phase peptide synthesis (LPPS) and solid phase peptide synthesis (SPPS). The LPPS method requires careful manipulation of protecting groups and multiple challenging post-treatments and separations, and is more suitable for shorter peptide and fragment synthesis. The SPPS method is currently the most commonly used peptide synthesis method, which uses resin as a protecting group to greatly simplify the separation and purification process of the product. Typical SPPS strategies include Boc peptide synthesis, Fmoc peptide synthesis, etc.
In Alfa Chemistry, we have automated synthesis platforms based on SPPS methods, such as high-throughput automated peptide synthesizers and microwave peptide synthesizers. Our automated synthesis platform can run 7×24h, which has maximized the efficient synthesis of peptides.
- Peptide Purification
Alfa Chemistry always adheres to the delivery of high-quality products and services, and we provide peptide purification and upgrading services. The purification techniques involved include, but are not limited to, high performance liquid chromatography (HPLC), supercritical fluid chromatography (SFC), size exclusion chromatography, ion exchange chromatography (IEC), partition chromatography.
Applications of Peptide Synthesis
Peptide synthesis has shown broad appeal in numerous fields, including biochemical research, drug development, diagnostics and therapeutics, industrial and agricultural applications, cosmetic and personal care industries, and even materials science.
Choose Alfa Chemistry
We understand that producing high-quality peptides can be a challenging and time-consuming process. That's why we offer best-in-class automated peptide synthesis services designed to make peptide production more efficient and accessible to researchers. Alfa Chemistry's automated peptide synthesis services are the optimal solution for researchers who require high-quality peptides in a timely and cost-effective manner. Our cutting-edge technology, experienced team, and strict quality control processes ensure that our Synthesized peptides meet the highest industry standards.
- Somesh Mohapatra, et al. ACS Cent. Sci. 2020, 6, 12, 2277-2286.
- Albert Isidro-Llobet, et al. J. Org. Chem. 2019, 84, 8, 4615-4628.